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. Author manuscript; available in PMC: 2010 Mar 30.
Published in final edited form as: Mol Cell. 2007 Aug 3;27(3):353–366. doi: 10.1016/j.molcel.2007.06.024

Figure 1. Expression Profile of the Igf2r Imprinted Cluster.

Figure 1

(A) Mouse embryo fibroblast cells (MEFB1 and MEFF) used to detect parental-specific expression and epigenetic modifications were prepared from 13.5 dpc +/Thp and Thp/+ embryos. +, wild-type chromosome 17. Thp, T hairpin chromosome 17 variant contains a 6 Mbp deletion including the 500 kb Igf2r imprinted cluster. The maternal chromosome is written on the left side.

(B) Top row, 380 kb map showing the Slc22a3, Slc22a2, Slc22a1, Igf2r, and Mas1 genes. The Air promoter lies in Igf2r intron 2. Air is a 108 kb single-exon ncRNA that overlaps Igf2r and Mas1 in antisense orientation. Bars, exons or pseudogenes. Thin arrows, silent genes in MEF cells. Thick arrows, maternally expressed gene (light gray), paternally expressed gene (dark gray). *L41 pseudogene, **Au76 pseudogene. The Mat Expression (MEFB1) and Pat Expression (MEFF) rows show cDNA hybridization to a custom PCR tiling array spanning three regions: 19 kb containing the Slc22a3 promoter, 13 kb containing the Slc22a2 promoter, and 220 kb from Slc22a1-3′ end to Mas1-3′ end (indicated by black bars underneath). Each column is the mean ratio of medians of eight replicate spots.