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. Author manuscript; available in PMC: 2010 Mar 30.
Published in final edited form as: Mol Cell. 2007 Aug 3;27(3):353–366. doi: 10.1016/j.molcel.2007.06.024

Figure 5. The Histone H3K9 Trimethylase ESET/SETDB1 Binds the Air and Igf2r HC Peaks.

Figure 5

(A) ESET ChIP-PCR at maternal and paternal alleles of the Air promoter (left), Igf2r promoter (middle), and Au76-psg (right), showing that ESET is present at Air and Igf2r alleles marked by H3K9me3. ESET is absent from Au76-psg and pericentric DNA (data not shown) assayed from the same ChIP preparation.

(B and C) (B) ChIP-PCR of the Air and Igf2r promoters and Au76-psg in wild-type control and (C) SUV39H null (SUV39H dn) diploid MEF cells (Peters et al., 2001) using antibodies for H3K9me3, H4K20me3, and HP1β. Increased signals were seen for HC peaks at the Air and Igf2r promoters, but only minimal changes were seen at Au76-psg. SUV39H dn MEFs maintain DNA methylation over the silent Air and Igf2r promoter regions (data not shown). H3K9me3 is absent from pericentric DNA assayed from the same SUV39H dn ChIP preparation (data not shown) as described (Schotta et al., 2004). *, weak reproducible signals.