Fig. 5.

Distribution of “label-retaining” cells at various periods after APAP “chase.” (A) The density of label-retaining cells in the smallest portal tract after APAP “chase” at days 0, 28, and 56. Label-retaining peribiliary hepatocytes and intralobular hepatocytes were decreasing for the first 4 weeks, then increasing during the next 4 weeks. During the 8-week follow-up, the density of label-retaining OVc was not changing statistically. (B) The distribution of label-retaining cells after APAP “chase” at days 0, 28, and 56. The percentage of label-retaining intralobular hepatocytes decreased between day 28 and day 56 statistically, whereas the percentage of label-retaining peribiliary hepatocytes increased during this same period. There was no statistical difference in the percentage of label-retaining OVc. These data suggest that intralobular hepatocytes are not “true” label retaining cells, losing label with time and normal hepatocyte turnover.