Figure 7. Variability of patchy LNs.
(a) Clustering of 161 patchy cell innervation patterns as in Fig. 3a. No two cells have identical innervation patterns.
(b) Schematic of MARCM-FLPout that allows two sister cells to be labeled by different colors. In this genetic method, UAS-FRT-CD2-FRT-mCD8GFP40 serves as a reporter of Gal4. After FLP-mediated mitotic recombination causes the loss of Gal80 in the ganglion mother cell (GMC), CD2 should be expressed in both daughter cells derived from this GMC. However, if an additional FLPout event occurs in one of the two daughter cells, this cell will express mCD8-GFP instead of CD2.
(c-h) Examples of two sister patchy cells (c-e) and two sister control cells (f-h) labeled by MARCM-FLPout shown with N-cadherin (blue), GFP (green) and CD2 (red) staining. (c, f) Projection of the entire antennal lobe. (d, e, g, h) High magnification of 5 μm projections from anterior (d, g) and middle (e, h) antennal lobe sections showing non-overlapping processes from 2 sister cells. Dashed lines mark the boundary of glomeruli VA1l/m (d), DL1 and DC2 (e). Scale bars, 20 μm.
(i) Sister cell overlap index as a function of edge length of the dilation kernel (see Methods). Dashed lines indicate the mean for patchy sister cells (right-shifted) and non-patchy sister cells (left-shifted). Colored lines indicate individual pairs of sister cells. A quantitative distinction between patchy and non-patchy classes was verified by K-means clustering into two clusters.