Table 1.
Analysis statistics of bacterioplankton community transcript libraries.
| Coastal 1 | Coastal 2 | Phyto-DOC | VP-DOC | |
|---|---|---|---|---|
| Number of unique reads | 435 219 | 291 109 | 405 024 | 444 832 |
| Average read length | 186 | 186 | 157 | 206 |
| Number of rRNAs | 230 108 | 150 603 | 230 432 | 225 696 |
| Number of potential proteins | 205 108 | 140 506 | 174 592 | 219 136 |
| Number of RefSeq hitsa | 68 327 | 65 700 | 107 196 | 144 514 |
| % RefSeq hitsb | 33 | 47 | 61 | 66 |
| Number of COG hitsc | 40 570 | 21 133 | 70 981 | 88 621 |
| % COG hitsb | 20 | 15 | 41 | 40 |
a
Criteria for annotation were E-value ≤ 0.01, amino acid identity ≥ 40% and overlapping length ≥ 23 aa to the corresponding best hit. See Experimental procedures for information on how these criteria were determined.
b
Expressed as a per cent of potential protein-encoding transcripts.
c
Criteria for annotation were E-value ≤ 0.1, amino acid identity ≥ 40% and overlapping length ≥ 23 aa to the corresponding best hit.
Coastal 1 and 2 = unmanipulated coastal DOC; Phyto-DOC = amended with DOC derived from four strains of coastal phytoplankton; VP-DOC = amended with DOC derived from senescent Spartina alterniflora, the dominant vascular plant in adjacent coastal marshes.