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Experimental Setup. Approximately 1 ml of 70% Glycerol was used as optical clearing agents. If ultrasound used with or without UCA, 1 MHz ultrasound transducer for 20 minutes span. In case UCA was applied to the experiment, 0.1 % concentration was chosen to use according to the guideline in UCA specification. After optical image was taken identical procedure was conducted twice more. Inner ring light sources were used to provide uniform luminance to the porcine skin sample.
