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Sequences and apparent melting temperatures (T1/2) of the oligonucleotides studied
| Name | Oligonucleotides |
|
|---|---|---|
| Sequence (5′–3′) | T1/2 (°C) | |
| AQ1 | d(TSGGGGT) | >100 |
| AQ2 | d(TGSGGGT) | 87 |
| AQ3 | d(TGGSGGT) | <25a; 75b |
| AQ4 | d(TGGGSGT) | 63 |
| AQ5 | d(TGGGGST) | >100 |
dS = dSpacer, tetrahydrofuranyl analog (Figure 1).
aEvaluated from the melting curve (1°C/min scan rate) after the annealing procedure.
bEvaluated from the melting curve after 1 week storage at 5°C.
