Table 3.

Kinetic parameters for the quadruplexes dissociation

ODN sequence	T (°C) ±1	koff × 104 (min−1)a	t1/2 (min)b	Eoff (kJ mol−1)
AQ2 d(TGSGGGT)	75	0.08 ± 0.04	8.7	131 ± 4
	80	0.16 ± 0.01	4.3
	85	0.37 ± 0.01	1.9
	90	0.81 ± 0.01	0.9
AQ4 d(TGGGSGT)	60	0.19 ± 0.04	3.6	166 ± 6
	63	0.30 ± 0.01	2.3
	65	0.40 ± 0.01	1.7
	67	0.50 ± 0.02	1.4

All the values have been determined in a 70 mM KCl, 10 mM KH₂PO₄, pH 7.0 buffer.

^ak_off is defined as d[S]/dt = −d[S₄]/dt = k_off[S₄].

^bt_1/2 is the half-dissociation time of quadruplex structures defined as