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. 2009 Dec 16;38(6):1997–2005. doi: 10.1093/nar/gkp1120

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© The Author(s) 2009. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Binding Assays for rMcrA-S. (A) Assays of electrophoretic mobility shifts with different molar ratios (1 : 1 and 1 : 4—DNA:protein) of rMcrA-S binding unmethylated and symmetrically methylated (m5CpG) containing 24-bp double-stranded oligonucleotides: 1 contains three m5C including one in an HpaII site; 2 contains a single m5C in a ‘three–fourth HpaII site’; 3 contains a single m5C in an HpaII site; 4 has no m5Cs. Molar ratios are based on monomeric McrA (16). Asterisks indicate the positions of McrA-S/DNA complexes. (B) Coomassie Blue staining of EMSA (A) gel.