Figure 9.

Hek293T cells were transiently co-transfected with plasmids encoding the chimeric Apobec3G WT or mutant constructs and the Apobec3G or VifSLQ prey constructs, combined with the pXP2d2-rPAP1-luci reporter. The transfected cells were either stimulated for 24 h with Epo or were left untreated (NS, not stimulated). Luciferase measurements were performed in triplicate. Data are expressed as fold induction (stimulated/NS). Western blot control of the different prey constructs in the experiment is shown.