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. 2009 Dec 18;38(6):1913–1921. doi: 10.1093/nar/gkp1162

Figure 3.

Figure 3.

Oxygen consumption measurement. Six and 26 months represent 24 and 22 cybrids per age group, respectively. Endogenous respiration was measured in intact cells (A), after treatment with the ATP synthase inhibitor oligomycin at 2.5 µg/ml (B), and with the uncoupler FCCP at 0.5 µM (C). The activities were determined with about 5 × 106 cells. Three determinations were made for each cybrid and the average values are shown. Ratios of oligomycin-resistant to native endogenous respiration rates (D) and FCCP-treated fully uncoupled to oligomycin-resistant respiration rates (E) of each age group were also calculated.