Figure 5.

Cell viability analysis. Six and 26 months represent 24 and 25 cybrids per age group, respectively. Cells were grown in glucose-containing DMEM for 24 h, then in glucose medium (A) or in galactose medium (B) for another 72 h. Cell viabilities were analyzed with a Vi-cell^TM XR cell viability analyzer (Beckman Coulter). Three determinations were made for each cybrid. Viability index was calculated as the ratio of percentage of cell viability in galactose-containing medium to that in glucose medium (C).