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. 2009 Dec 18;38(6):1913–1921. doi: 10.1093/nar/gkp1162

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© The Author(s) 2009. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — mtDNA copy number analysis. (A) Six and 26 months represent 24 and 20 cybrids per age group. Relative mtDNA copy number (mtDNA/nDNA) was the mtDNA content normalized by nuclear DNA encoded β-actin gene. (B) Relationship between the relative mtDNA copy number and the maximal respiration as measured by oxygen consumption with uncoupler FCCP in 40 individual transformants of both age groups. (C) Relationship between the relative mtDNA copy number and the ratio of doubling time (in galactose/in glucose media) in 40 individual transformants of both age groups. (D) Relationship between the relative mtDNA copy number and the ratio of viability (in galactose/in glucose media). In (B–D), n includes 22 cybrids of 6-month-young mice, and 18 cybrids of 26-month-old mice.