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. 2009 Dec 22;38(6):e87. doi: 10.1093/nar/gkp1163

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© The Author(s) 2009. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — The UDG/endoVIII repair protocol applied to mammoth DNA. (a) A mammoth DNA extract was subjected to 454 adaptor ligation after enzymatic repair in replicates in three conditions: (1) Incubation with PNK followed by T4 polymerase; (2) PNK and UDG followed by T4 polymerase; (3) PNK, UDG and endoVIII followed by T4 polymerase. The resulting libraries were quantified by qPCR. The same reactions without PNK were applied to mammoth DNA that had first been dephosphorylated by CIP (treatments 4–6). (b) All libraries were sequenced on 454. The proportions of sequences that aligned to the elephant genome are shown. (c) For the sequences resulting from treatments 1–3 and 6 the base composition of aligned elephant sequences is shown for 10 bases either side of the 5′-start point of mammoth sequences.