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. 2009 Dec 21;38(6):1832–1840. doi: 10.1093/nar/gkp1165

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© The Author(s) 2009. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Ascorbate protects against oxidation. (A) Protection of 6-TG against photochemical destruction. The 6-TG (0.1 mM in aqueous solution) was irradiated with the doses shown in the presence of different concentrations of ascorbate. Photochemical destruction of 6-TG was monitored by the reduction in A₃₄₂ spectrophotometrically. (open circle) no ascorbate (filled circle) ascorbate:6-TG 3: 1 (filled square) 12: 1 (open square) 25: 1 (filled triangle) 100: 1. (B) Protection of DNA 6-TG. A 11-mer oligonucleotide (1 µM) (CAGXAATTCGC where X = 6-TG) was UVA irradiated in the presence of ascorbate as indicated; 0 µM ascorbate (filled square), 10 µM (open circle) or 20 µM (filled circle). Conversion of 6-TG to G^SO3 in the intact oligonucleotide was monitored fluorimetrically (λ_ex 320 nm; λ_em410 nm).