Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Dec 21;38(6):2006–2018. doi: 10.1093/nar/gkp1171

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2009. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 4. — Activity of DmoCre mutants with altered specificity in both I-DmoI and I-CreI subdomains. (a) Extrachromosomal assay in CHO-K1 cells. Cells were transfected with different meganucleases or an empty vector and the cleavage against the two D2-Ca and D(4AGT)-Ca (targets was measured). The nucleotides in the D(4AGT)-Ca target for which the specificity has been modified have been gray boxed. (b) Extrachromosomal assay in CHO-K1 cells. Cells were transfected with different meganucleases or an empty vector (mock) and the cleavage against the two D2-Cb and D(4AGT)-Cb targets was measured. The nucleotides in the D(4AGT)-Cb target for which the specificity has been modified have been gray boxed.