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. 2009 Dec 21;38(6):2006–2018. doi: 10.1093/nar/gkp1171

Table 1.

Cleavage activity of DmoCre variants with altered specificity within the I-CreI moiety

Targets derived from C1221 that are cleaved by I-CreI mutantsa Number and strength of the I-CreI mutants Targets cleaved by DmoCre mutantsa, number of positive DmoCre mutants and maximal cleavage activity
CAAAACTTGGTACCAAGTTTTG 16 0.11 < AU < 0.30 CGCCGGAACTTACCAAGTTTTG 16 AUmax = 0.56
CAAAACGAGGTACCTCGTTTTG 36 0.2 < AU < 1.0 CGCCGGAACTTACCTCGTTTTG 32 AUmax = 0.92
CCCAACGTCGTACGACGTTGGG 14 0.15 < AU < 0.70 CGCCGGAACTTACGACGTTGGG 12 AUmax = 0.92
CTGGCTGCTGTACAGCAGCCAG 26 0.61 < AU < 0.94 CGCCGGAACTTACAGCAGCCAG 19 AUmax = 0.89
CGTTCTCAGGTACCTGAGAACG (Ca) 33 0.4 < AU < 1.0 CGCCGGAACTTACCTGAGAACG (D2-Ca) 8 AUmax = 0.40; (0.91)b
CTGGCTGAGGTACCTCAGCCAG (Cb) 35 0.2 < AU < 0.74 CGCCGGAACTTACCTCAGCCAG ( D2-Cb) 6 AUmax = 0.48; (0.97)b

AU: arbitrary unit.

aBases bold underlined are bases for which the I-CreI specificity has been modified.

bCleavage activity after protein optimization.