Table 3.
Cleavage activity in yeast of DmoCre variants with altered specificity in both I-DmoI and I-CreI moieties
| Library template (target specificity) | Randomized positions | Target tested | Maximal cleavage activity observed in yeast (AU value) |
|---|---|---|---|
| Vb (D2-Cb) | 75, 76, 77 | D(4AGT)-Cb | 0.91 |
| D(4ATA)-Cb | 0.57 | ||
| D(4AGA)-Cb | 0.66 | ||
| D(4AGG)-Cb | 0.50 | ||
| 29, 33, 35 | D(10CTC)-Cb | 0.19 | |
| D(10TAG)-Cb | 0 | ||
| D(10GCG)-Cb | 0.64 | ||
| D(10ACG)-Cb | 0.64 | ||
| D(10CCA)-Cb | 0.41 | ||
| Va (D2-Ca) | 75, 76, 77 | D(4AGT)-Ca | 0.87 |
| D(4ATA)-Ca | 0 | ||
| D(4AGA)-Ca | 0.34 | ||
| D(4AGG)-Ca | 0.37 | ||
| 29, 33, 35 | D(10CTC)-Ca | 0 | |
| D(10TAG)-Ca | 0 | ||
| D(10GCG)-Ca | 0.43 | ||
| D(10ACG)-Ca | 0 | ||
| D(10CCA)-Ca | 0.2 |