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. 2009 Dec 21;38(6):1805–1820. doi: 10.1093/nar/gkp1172

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© The Author(s) 2009. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Co-occupancy by TFIID and SAGA at the promoters of several class II genes. Strains expressing HA-tagged TAF1 (YTK2741) alone or in combination with PK-tagged TAF8 (YTK6824), GCN5 (YTK6831), SUA7 (YTK6837), TFA2 (YTK6838), TFG1 (YTK6839) or TFB3 (YTK6840) were cultured and cross-linked as described in Figure 1. The cross-linked chromatin was prepared and precipitated with anti-HA monoclonal antibody. The eluates were then re-precipitated with anti-CTD (Rpb1, G) or anti-PK (Taf8, A; Gcn5, B; Sua7, C; Tfa2, D; Tfg1, E; Tfb3, F) monoclonal antibodies. After the cross-link reversal, quantitation of the ratio of DNA recovered after the second immuno-precipitation step to the input was performed as described in Figure 2. The data from three independent experiments are presented as mean ± SD.