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. 2009 Dec 29;38(6):e88. doi: 10.1093/nar/gkp1181

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© The Author(s) 2009. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Replication and structure of phage N15 and linear pJAZZ vectors. Left panel: Replication of phage N15 and the pJAZZ vectors requires the phage proteins RepA and TelN. Bidirectional replication is initiated by RepA within the repA gene. TelN cleaves the newly replicated telomeres to re-create the closed hairpin ends. Right panel: The lysis and structural genes of N15 were deleted to create pG591. The vector pJAZZ-KA was derived from pG591 by insertion of a lacZ-alpha ‘stuffer’ fragment, situated between a pair of identical multiple cloning sites. A drug resistance gene was added to the right arm. The vectors pJAZZ-OK, pJAZZ-OC and pJAZZ-OCmin were derived from the pJAZZ-KA vector. The lacZ-alpha stuffer is removed before ligation to target DNAs. Transcription is indicated by arrows and transcriptional terminators by ‘T’. Dark balls represent the hairpin telomeres; cB, regulator of replication; MCS, Multiple Cloning Site (SmaI, NotI, AflII … LacZ-alpha … AflII, NotI, SmaI).