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Assembly of the Flavobacterium genome (69% AT) in the pJAZZ vector. Fragments of 2–6 kb, 6–10 kb and 10–12 kb were cloned into the pJAZZ-KA vector. Clones were end-sequenced to provide ∼10X genomic coverage. Assembly of pJAZZ libraries of this AT-rich genome closely approximated the predicted Lander–Waterman curve.
