Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Dec 29;38(6):e89. doi: 10.1093/nar/gkp1182

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2009. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 5. — Quantities and qualities of the recovered E. coli under various hybridization conditions. (A) Recovered amounts of the tRNA from 5 A₂₆₀ units of unfractionated E. coli tRNAs at various hybridization temperatures. (B) Analysis by 10% PAGE containing 7 M urea. Lane mix: 0.1 A₂₆₀ units of unfractionated E. coli tRNAs. Lane Au: 0.01 A₂₆₀ units of the authentic E. coli . Lanes Na⁺, TMA⁺ and TEA⁺: 0.01 A₂₆₀ units of the recovered E. coli with hybridization buffer Na⁺ (0.9 M NaCl, hybridized at 65°C), hybridization buffer TMA⁺ (TMA-Cl, hybridized at 65°C) and hybridization buffer TEA⁺ (TEA-Cl, hybridized at 35°C), respectively. The gel was stained with methylene blue. The asterisk shows a contaminant band found in the recovered E. coli . (C) Northern hybridization to detect E. coli in the tRNA fractions unbound to the resin. (Left) Lane mix: 0.1 A₂₆₀ units of unfractionated E. coli tRNAs. Lanes Na⁺, TMA⁺ and TEA⁺: 0.1 A₂₆₀ units of the tRNA fractions unbound to the resin using hybridization buffer Na⁺ (hybridized at 65°C), hybridization buffer TMA⁺ (hybridized at 65°C) and hybridization buffer TEA⁺ (hybridized at 35°C), respectively. The gel was stained with methylene blue. (Right) Northern hybridization analysis with Oligo-EfMet. Biotins on the nylon membrane were detected with streptavidin, bitotinyl alkaline phosphatase and BCIP/NBT substrate.

Inline graphic — Quantities and qualities of the recovered E. coli under various hybridization conditions. (A) Recovered amounts of the tRNA from 5 A₂₆₀ units of unfractionated E. coli tRNAs at various hybridization temperatures. (B) Analysis by 10% PAGE containing 7 M urea. Lane mix: 0.1 A₂₆₀ units of unfractionated E. coli tRNAs. Lane Au: 0.01 A₂₆₀ units of the authentic E. coli . Lanes Na⁺, TMA⁺ and TEA⁺: 0.01 A₂₆₀ units of the recovered E. coli with hybridization buffer Na⁺ (0.9 M NaCl, hybridized at 65°C), hybridization buffer TMA⁺ (TMA-Cl, hybridized at 65°C) and hybridization buffer TEA⁺ (TEA-Cl, hybridized at 35°C), respectively. The gel was stained with methylene blue. The asterisk shows a contaminant band found in the recovered E. coli . (C) Northern hybridization to detect E. coli in the tRNA fractions unbound to the resin. (Left) Lane mix: 0.1 A₂₆₀ units of unfractionated E. coli tRNAs. Lanes Na⁺, TMA⁺ and TEA⁺: 0.1 A₂₆₀ units of the tRNA fractions unbound to the resin using hybridization buffer Na⁺ (hybridized at 65°C), hybridization buffer TMA⁺ (hybridized at 65°C) and hybridization buffer TEA⁺ (hybridized at 35°C), respectively. The gel was stained with methylene blue. (Right) Northern hybridization analysis with Oligo-EfMet. Biotins on the nylon membrane were detected with streptavidin, bitotinyl alkaline phosphatase and BCIP/NBT substrate.