Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Dec 30;38(6):2044–2056. doi: 10.1093/nar/gkp1187

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2009. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 4. — TnpI-mediated recombination at the reversed core site IRS2.1 variant in vitro. Supercoiled plasmids carrying directly repeated copies of wild-type IRS (IRS1.2 × 1.2) or modified IRS in which the orientation of the IR1–IR2 core site is reversed with respect to the DR1–DR2 accessory sequence (IR2.1 × 2.1) were incubated for the indicated times with purified TnpI. Reaction were digested with AvaII (AII) and analysed by electrophoresis on a 0.8% agarose gel. S1, S2 and S3 are the AvaII fragments from the initial substrates. D1 and D2 are specific fragments from the deletion products. AvaII digestion of the reactions also produced χ-forms corresponding to the Holliday junction (HJ) intermediate of recombination.