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Representative recombineering efficiency as a function of insert size. 1000–4500-bp inserts containing the neo gene and bearing 50-bp flanking homology regions were inserted into the lacZ gene of strain K-12 MG1655 pTKRED via the method of Datsenko and Wanner (6). Cells were plated on LB agar + 25 μg/ml kanamycin, and the number of successful recombinants quantified as the number of resulting white colonies.
