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pTKIP is cured by in vivo I-SceI cleavage. Circles indicate the number of cells retaining pTKRED, squares indicate retention of pTKIP-neo. Normalized cfu is the ratio of surviving colonies on plates containing the appropriate antibiotic (100 μg/ml spectinomycin and 25 μg/ml kanamycin for pTKRED and pTKIP, respectively) to surviving colonies on LB plates without selection.
