Table 1.
Selectionless insertion statistics for insertion of the 7-kb IvT-O1Z-neo cassette at the indicated positions
| Insertion location | IPTG (λ-Red induction) | Total (blue/white) | % Integrants |
|---|---|---|---|
| atpI-gidB | + | 705/4 | 99.4 |
| − | 1010/44 | 95.8 | |
| yieN-trkB | + | 642/15 | 97.7 |
| − | 461/163 | 73.9 | |
| ygcE-ygcF | + | 39/100 | 28.6 |
| − | 184/1084 | 14.5 | |
| ybbd-ylbG | + | 178/133 | 57.2 |
| − | 354/2295 | 13.4 | |
| nth-ydgR | + | 191/0 | 100 |
| − | 362/16 | 95.8 | |
| essQ-cspB | + | 377/1583 | 19.2 |
| − | 43/685 | 5.9 |
Samples were plated on LB agar plates +0.1% X-Gal, 2 mM IPTG without antibiotic selection; these plates were replica printed onto LB agar plates with 25 μg/ml kanamycin, 100 μg/ml ampicillin or 10 μg/ml tetracycline.
% integrants: the number of blue colonies displaying appropriate antibiotic sensitivities divided by total number.