(A and B) 7-week-old KO mice (n = 5) or control littermates (n = 10) were administered i.p. with 0.5 mg LPS/mouse. Mice were bled at 0 (before LPS injection), 1.5, 3, and 6 hr after LPS injection. Serum IL-12p40 (A, top), IL-6 (A, bottom), and TNF-α (B) were quantified by ELISA. *p < 0.05; **p < 0.01. Two experiments were performed with similar findings.
(C) Survival curve of KO (n = 12) or WT (n = 27) mice after i.p. injection of 0.5 mg LPS/mouse.
(D and E) WT (n = 6) and KO (n = 5) mice were injected i.v. with 1 mg LPS/mouse, followed by measurement of serum TNF-α (D) and IL-12p40 (E).