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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Deep Sea Res 2 Top Stud Oceanogr. 2010 Feb 1;57(3-4):279–287. doi: 10.1016/j.dsr2.2009.09.006

Fig. 3.

Fig. 3

Representative qPCR standard curve. Standard curves were constructed from serial dilutions of a plasmid containing a cloned fragment of the large subunit ribosomal RNA gene of A. tamarense. Reaction efficiencies ranged from 86–100% over all runs. The lowest target copy number tested was 520 copies per reaction, which corresponds to a detection limit of 10 cysts/cc in a standard sample volume of 4cc.