Figure 1.
TfR2 expression in GBM cell lines. (A) Flow cytometry analysis of TfR1 and TfR2 expression in TB10, U87MG, T89G, and U251 GBMcell lines and in BTSC1 and BTSC83 GBM stem cell lines. Cells have been labeled with anti-TfR1 and anti-TfR2 mAbs and then analyzed by flow cytometry. (B) Western blot analysis of TfR1 and TfR2 expression in TB10, U87GM, T98G, and U251 GBM cell lines and in BTSC1 and BTSC83 GBM stem cell lines. Blots were stripped and reprobed for actin to ensure equivalent loading and transfer. (C and D) Association of TfR2 with lipid rafts/caveolae by density gradient centrifugation. TB10 (C) and U251 (D) cells were lysed in Triton X-100 and subjected to sucrose gradient centrifugation. Aliquots of fractions collected from the top of the gradient were analyzed by Western blot analysis with the use of antibodies against TfR1, TfR2, and caveolin-1 (Cav-1). Equal protein amounts for each fraction were loaded on SDS-PAGE.