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. 2010 Apr;3(2):123–134. doi: 10.1593/tlo.09274

Figure 4.

Figure 4

Effect of hypoxia on TfR2 expression. (A) Flow cytometry analysis of TfR2 expression in TB10 cells grown for 40 hours either in the absence (C) or in the presence of 100 µM CoCl2. (B) Western blot analysis of HIF-1α expression in U251 cells grown for either 24 or 48 hours either in the absence (C) or in the presence of 100 µM CoCl2 and in TB10 cells grown for 48 hours under normal (20% O2) and reduced (5% and 1% O2) oxygen tension. (C) Analysis of the level of fluorescence (mean fluorescence intensity values normalized with respect to their negative control) of TfR2 labeling observed in TB10 and U251 cells grown for either 24 or 48 hours either in the absence (C) or in the presence of 100 µM CoCl2 or under controlled (20%, 5%, and 1% O2) oxygen tension. Data represent the mean values ± SEM observed in three independent experiments. *P < .05, **P < .01. NS indicates not significant. (D) Western blot analysis of TfR2 expression in U251 and TB10 cells grown for 24 or 48 hours either in the absence (C) or in the presence of 100 µM CoCl2 or under controlled (20%, 5%, and 1%O2) oxygen tension. (E) TfR2 levels in TB10 and U251 cells grown under controlled (20%, 5%, and 1% O2) oxygen tension have been determined by densitometry of Western blot autoradiograms and, after normalization with respect to actin, have been expressed in arbitrary units (AU) and then plotted.

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