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A segment of the ANTXR1-sv1 cytoplasmic domain binds actin. Peptides TAIL360-420 and TAIL360-420(Y383A) were bound to streptavidin beads and incubated with (A) HeLa cell lysates or (B) with purified β-actin. The beads were pelleted and the associated proteins were subjected to SDS-PAGE and tranferred to nitrocellulose. Blots were probed for biotin, to detect the peptides, or for β-actin. Blots are representative of three independent experiments.
