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Schematic diagrams of gold nanoparticle (AuNP) probe synthesis and activation. (a) One-step reaction method for generating self-assembled AuNP probes with different surface compositions, consisting of Quasar 670-labeled and BHQ-2-labeled peptide substrates as well as SH-PEG. (b) Proposed mode of AuNP probe activation by target protease in its conversion from a dark quenched to a near-infrared fluorescent state.
