Table 3.
N-glycosylated peptides identified from lectin affinity enriched proteins of HCC tissue
| RefSeq accession number | Protein name | Expect value | Mascot score | Peptide sequence | Known? | Consensus motif |
|---|---|---|---|---|---|---|
| NP_000005.2 | Alpha-2-macroglobulin | 1.0E–01 | 42 | SLGNV*NFTVSAEALESQELCGTEVPSVPEHGRK | YES | NFT |
| 2.1E–03 | 57 | VS*NQTLSLFFTVLQDVPVR | YES | NQT | ||
| NP_000375 | Apolipoprotein B | 8.3E–05 | 62 | F*NSSYLQGTNQITGR | YES | NSS |
| NP_001638.1 | Apolipoprotein D | 7.2E–02 | 42 | ADGTVNQIEGEATPV*NLTEPAKLEVK | YES | NLT |
| P00450 | Ceruloplasmin | 3.4E–06 | 85 | E*NLTAPGSDSAVFFEQGTTR | YES | NLT |
| NP_001822.2 | Clusterin isoform 1 | 2.1E–05 | 76 | LA*NLTQGEDQYYLR | YES | NLT |
| NP_009224.2 | Complement component 4A | 1.3E–02 | 47 | GL*NVTLSSTGR | NO | NVT |
| NP_001747.2 | Corticosteroid binding globulin | 3.4E–04 | 54 | AQLLQGLGF*NLTER | YES | NLT |
| NP_005132.2 | Fibrinogen, beta chain | 9.5E–05 | 59 | GTAGNALMDGASQLMGE*NR(T) | NO | NRT |
| NP_000500.2 | Fibrinogen, gamma chain isoform gamma-A | 1.5E–06 | 78 | DLQSLEDILHQVE*NK(T) | YES | NKT |
| NP_006673.1 | Fibrinogen-like 2 | 9.9E–02 | 38 | LDGST*NFTR | NO | NFT |
| 7.1E–05 | 62 | LHVGNY*NGTAGDALR | NO | NGT | ||
| NP_997640.1 | Fibronectin 1 isoform 2 | 4.3E–02 | 44 | DQCIVDDITYNV*NDTFHK | YES | NDT |
| NP_997640 | Fibronectin 1 isoform 2 | 8.9E–07 | 82 | LDAPTNLQFV*NETDSTVLVR | NO | NET |
| NP_009201 | FK506 binding protein 9 | 1.1E–03 | 51 | YHYNASLLDGTLLDSTWNLGK | NO | NAS |
| NP_005657.1 | H factor (complement)-like 3 | 6.5E–05 | 61 | LQNNEN*NISCVER | NO | NIS |
| NP_005134 | Haptoglobin | 1.5E–05 | 78 | MVSHH*NLTTGATLINEQWLLTTAK | YES | NLT |
| 3.5E–07 | 85 | NLFL*NHSENATAK | YES | NHS | ||
| 2.9E–06 | 71 | VVLHP*NYSQVDIGLIK | YES | NYS | ||
| NP_000604 | Hemopexin | 2.1E–05 | 76 | ALPQPQ*NVTSLLGCTH | YES | NVT |
| 3.5E–08 | 94 | SWPAVG*NCSSALR | YES | NCS | ||
| NP_000403.1 | Histidine-rich glycoprotein | 5.6E–03 | 52 | VIDF*NCTTSSVSSALANTK | YES | NCT |
| NP_002207 | Inter-alpha globulin inhibitor H2 | 2.3E–02 | 37 | GAFISNFSMTVDGK | YES | NFS |
| NP_000884 | Kininogen 1 isoform 2 | 5.4E–06 | 73 | ITYSIVQTNCSK | NO | NCS |
| NP_071751 | Leucine proline-enriched proteoglycan 1 | 2.7E–03 | 47 | VPLQSAHLYYNVTEK | NO | NVT |
| NP_006491 | Melanoma cell adhesion molecule | 7.2E–03 | 42 | CVASVPSIPGLNR(T) | NO | NRT |
| NP_002395 | Microfibrillar-associated protein 4 | 2.3E–04 | 57 | FNGSVSFFR | NO | NGS |
| 1.1E–04 | 69 | VDLEDFE*NNTAYAK | NO | NNT | ||
| NP_006380.1 | Oxygen regulated protein | 3.2E–09 | 104 | LSALDNLL*NHSSMFLK | YES | NHS |
| CAJ15161 | Alpha-antitrypsin | 4.0E–08 | 102 | YLG*NATAIFFLPDEGK | YES | NAT |
| NP_000479.1 | Serine (or cysteine) proteinase inhibitor, clade C | 8.1E–06 | 70 | LGAC*NDTLQQLMEVFK | YES | NDT |
| NP_001076.2 | serpin peptidase inhibitor, clade A | 2.3E–04 | 65 | F*NLTETSEAEIHQSFQHLLR | NO | NLT |
| NP_001031.2 | Sex hormone-binding globulin | 9.5E–03 | 41 | LDVDQAL*NR(S) | NO | NRS |
| NP_001054 | Transferrin | 7.4E–06 | 72 | CGLVPVLAENY*NK(S) | YES | NKS |
| 3.0E–02 | 46 | QQQHLFGS*NVTDCSGNFCLFR | YES | NVT |
Accession number and protein names were from NCBI RefSeq database, and mascot score and expect values were listed for N-glycosylated peptides. Human protein reference database (www.hprd.org) was used for the identification of previously reported N-glycosylation sites. An asterisk in *N-X-T/S implies 18 O labeling of Asn after PNGase F mediated cleavage of glycan.