Figure 2.
The combination of CY and PC61 given concurrently with the GM‐CSF whole‐cell vaccine is more effective in augmenting antitumor activity when compared with CY or PC61 administered as single agents with the vaccine. C57BL/6 mice were challenged on day 0 with 2.5 × 105 Panc02 tumor cells, as described, followed by administration of 100 mg/kg of CY or 50 μg of PC61 on day 2 and irradiated whole‐cell vaccine on day 3. Mice were treated with vaccine alone (A), vaccine plus CY (·), vaccine plus PC61 (♦), or vaccine plus CY and PC61 (▾). Identical groups were set up with mock vaccine administered as a control (open symbols). Mice left untreated following inoculation of tumor (▪) served as a positive control. The data are presented as Kaplan‐Meier survival curves that indicate the percentage of tumor‐free animals as a function of time after tumor inoculation. Data are represented as results obtained from experiments with at least 10 mice per group. Tumor incidence in mice that received CY and PC61 in addition to vaccine was significantly different by the log‐rank test, when compared with animals that received tumor alone (p < 0.0001), PC61 plus vaccine (p < 0.0001), or CY plus vaccine (p = 0.0006).