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. Author manuscript; available in PMC: 2010 Mar 30.
Published in final edited form as: Biochemistry. 2009 Nov 24;48(46):10956–10962. doi: 10.1021/bi900978f

Figure 6.

Figure 6

PDZ-kinase and reengineered Cas restore migration defect of Cas-deficient cells. Migration assays on Cas–/– cells expressing the indicated constructs were carried out in a 96-well transwell migration assay chamber. Expression of kinases and Cas variants was confirmed by Western blotting (Supplemental Figure 4, Supporting Information). Migration experiments were also carried out with Cas-VKESLV, a Cas construct that contains a Src binding sequence with an intact polyproline sequence plus the PDZ ligand at the C-terminus.