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. 2010 Apr 15;137(8):1315–1325. doi: 10.1242/dev.043190

Fig. 6.

Fig. 6.

Nectin-2 interacts with N-cadherin, enhancing apical constriction. (A) A GST pull-down assay with nec2-ex and Ncad-ex, Ecad-ex or Ccad-ex performed using the 293T cell line. (B-G) Overexpression of nectin-2 or N-cadherin in non-neural ectoderm. Low doses of nectin-2 (50 pg; B,C) or N-cadherin (50 pg; D,E) alone and in combination (F,G) were injected and observed at stages 13-14 (early neurulae). Arrows: apically constricted cells. (H) Embryos with aberrant surface in the overexpression experiments with 50 pg of nectin-2 and 50 pg of N-, E- or C-cadherin. Error bars: mean ± s.e.m. (I) Nectin-2 (50 pg) and N-cadherin (50 pg) were separately injected into adjacent blastomeres of four-cell-stage embryos with memGFP and memRFP, respectively. The injected embryos were observed at the early neurula stage. (J-M) Overexpression of E- (J,K) or C-cadherin (L,M) with or without 50 pg of nectin-2. Arrows: apically constricted cells. Scale bars: 50 μm.