Fig. 2.

The 1/2 and 4/5 boundaries align before their neighbors and ush mutants align normally. (A) Alignment by stage. (B) Stage 13 denticle field with columns marked by number and column boundaries marked by color. At stages 12 and 13, the 1/2 and 4/5 boundaries are significantly better aligned than the 2/3, 3/4 and smooth boundaries. At stages 14 and 15, all columns of the denticle field are significantly better aligned than the smooth field boundary. (C) Alignment by column, comparing ush mutants (muts) with ush heterozygotes (hets). (D,E) Anti-phosphotyrosine (pY)-stained embryos. Denticle field alignment is nearly identical in homozygous ush mutants (E) and ush/+ (D).