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. 2010 Apr 15;137(8):1385–1394. doi: 10.1242/dev.048520

Fig. 3.

Fig. 3.

pY, F-actin and Zip are enriched along the column 1/2 and 4/5 boundaries. Immunofluorescence for pY, Patched (Ptc), Serrate (Ser), F-actin and Zip. Anterior is to the left. Roughly two parasegments are visible. (A-C) PtcGal4 UAS-GFP embryos stained for (A) pY and (B) GFP (C, merge; GFP, green). Arrows mark pY enrichments at the 1/2 boundary between Ptc-expressing and non-expressing cells. (D-F) Ser-lacZ-expressing embryos stained for (D) pY and (E) for β-galactosidase by antibody (F, merge; lacZ, green). Arrows mark pY enrichments at the 4/5 boundary between Ser-expressing and non-expressing cells. (G-I) P{sqh-Moe.GFP}-expressing embryos stained for (G) pY and (H) GFP (I, merge; GFP, green). Arrows mark pY and F-actin enrichments at the 1/2 and 4/5 boundaries. Arrowheads mark occasional, variably positioned enrichments. (J-L) Embryos stained for (J) pY and (K) Zip (L, merge; Zip, magenta). Arrows mark pY and Zip enrichments at the 1/2 and 4/5 boundaries. Arrowheads mark occasional, variably positioned enrichments. Scale bar: 5 μm.