Table I.
Comparison of mediator production by CD34+ cell-derived and monocyte-derived DCs in response to EDN or hPRa
| CD34+ Cell-Derived DCs |
Monocyte-Derived DCs |
|||
|---|---|---|---|---|
| Mediatorsb | EDN | hPR | EDN | hPR |
| ENA-78 | 91 | 26 | 2 | 1 |
| Eot2 | 1 | 1 | 3 | 3 |
| I-309 | 4 | 3 | 4 | 1 |
| IFN-α | 1 | 1 | 5 | 0 |
| IL-10 | 1 | 2 | 6 | 0 |
| IL-12p40 | 32 | 4 | 3 | 1 |
| IL-12p70 | 7 | 2 | 2 | 1 |
| IL-13 | 1 | 2 | 3 | 1 |
| IL-16 | 1 | 1 | 0 | 3 |
| IL-6 | 182 | 74 | 181 | 15 |
| IP-10 | 74 | 10 | 67 | 6 |
| MCP-1 | 4 | 4 | 2 | 1 |
| MCP-2 | 26 | 8 | 18 | 1 |
| MCP-3 | 97 | 18 | 1 | 1 |
| M-CSF | 5 | 2 | 1 | 1 |
| MIG | 4 | 2 | 6 | 1 |
| MIP-1α | 14 | 7 | 10 | 2 |
| MIP-1β | 2 | 2 | 61 | 19 |
| MPIF-1 | 3 | 2 | 6 | 6 |
| NAP-2 | 4 | 2 | 2 | 1 |
| RANTES | 22 | 12 | 44 | 7 |
| sCD23 | 2 | 3 | 1 | 1 |
| TNF-α | 25 | 5 | 10 | 1 |
| sTNF-RI | 3 | 2 | 1 | 2 |
CD34+ cell-derived and monocyte-derived iDCs were cultured at 37°C in humidified air containing 5% CO2 in G4 medium alone or G4 medium plus EDN or hPR (both at 1 μg/ml) for 48 h. The culture supernatants were collected and stored at −70°C. The level of cytokines, chemokines, and soluble receptors (a total of 78) in each supernatant was measured by RCA immunoassay and presented as fold increase, which was calculated by the following formula: fold increase = Cy5 fluorescence in the presence of EDN or human pancreatic RNase/Cy5 fluorescence in the absence of EDN or hPR. Only those with fold increase ≥3 by EDN or hPR are shown.
MPIF, Myeloid progenitor inhibitory factor; NAP, neutrophil-activating protein; s, soluble.