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. Author manuscript; available in PMC: 2010 Mar 30.
Published in final edited form as: J Immunol. 2010 Jan 6;184(3):1507–1515. doi: 10.4049/jimmunol.0901219

FIGURE 6.

FIGURE 6

BEL does not stimulate apoptosis in neutrophils. A, Neutrophils (10 ± 106/ml) were incubated with PBS, DMSO (0.1%), and BEL (1– 20 μM) for 15 min at 37°C. Cells were labeled with Annexin V or PI to detect apoptotic and necrotic neutrophils. The upper right quadrant of the dot blot represents Annexin V- and PI-positive cells. The histogram represents Annexin V-positive cells at a threshold setting of 102 FL1-H. B, Annexin V-positive cells were counted and are represented on a bar graph, and data are represented as percent cells positive and as mean ± SEM for n = 3.