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. Author manuscript; available in PMC: 2010 Mar 30.
Published in final edited form as: J Immunol. 2010 Jan 6;184(3):1507–1515. doi: 10.4049/jimmunol.0901219

FIGURE 7.

FIGURE 7

BEL does not inhibit the phosphorylation of p47phox. Neutrophils (25 × 106/ml) were incubated with or without BEL (20 μM) and then stimulated with 1 μM fMLP for 30, 60, or 180 s. The reaction was stopped by adding ice-cold lysis buffer, and samples were prepared for Western blotting. Equal amounts of protein (10 μg/lane) were loaded on 10% SDS-PAGE gels and transferred to PVDF membranes. Membranes were then probed with anti-PKC phosphoserine substrate Ab (1:500). The membranes were stripped and reprobed with anti-p47phox (1:500). Data were obtained by a densitometry, and the results are presented as phospho-p47phox/total p47phox ratio. Results are expressed as mean ± SEM for n = 3.