Figure 2.
Wsc1-L63R is degraded rapidly using an ERAD-independent pathway. (A) Wild-type cells expressing Wsc1p or Wsc1-L63R were pulse-labeled at 30°C with [35S]methionine/cysteine for 10 min followed by a cold chase for times indicated. Proteins were resolved by SDS-PAGE and quantified by phosphorimager analysis. The data plotted reflect three independent experiments with mean ± SD indicated. (B) Wsc1-L63R turnover was measured in wild-type, Δcue1, Δhrd1, and Δdoa10 strains by pulse-chase analysis as described in A.