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. 2010 Apr 1;21(7):1178–1187. doi: 10.1091/mbc.E09-03-0229

Figure 1.

Figure 1.

STX6 interacts with CAL and promotes CFTR degradation. (A) HEK293 cells were cotransfected with 3 μg myc-CAL and 3 μg GFP-CFTR with or without 6 μg HA-STX6 as indicated. Forty-eight hours after transfection, cell lysates were harvested and immunoprecipitated by HA-affinity matrix. HA-STX6 interacted with both myc-CAL and GFP-CFTR. (B) HEK293 cells were cotransfected with 3 μg GFP-CFTR and increasing amount of HA-STX6 (3, 6, and 9 μg as indicated). Forty-eight hours after transfection, cell lysates were harvested and subjected to Western blotting analysis. HA-STX6 in a dose-dependent manner reduced GFP-CFTR protein expression. (C) HEK293 cells were cotransfected with 3 μg GFP-CFTR and HA-Vti1b (3, 6, and 9 μg as indicated). Forty-eight hours after transfection, cell lysates were harvested and subjected to Western blotting analysis. HA-Vti1b had no effect on GFP-CFTR protein expression. (D) HEK293 cells were cotransfected with 3 μg GFP-CFTR and STX16 (3, 6, and 9 μg as indicated). At 48 h after transfection, STX16 had no effect on GFP-CFTR protein expression. In these and all subsequent experiments, data shown are representative of at least three independent experiments.