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. 2010 Apr 1;21(7):1178–1187. doi: 10.1091/mbc.E09-03-0229

Figure 8.

Figure 8.

Silencing of STX6 in CF epithelial cell line CFBE-ΔF508CFTR. (A) CFBE-ΔF508CFTR cells were transfected with 20 nM STX6 siRNA or 20 nM control siRNA as indicated. Seventy-two hours after the transfection, cell lysates were collected and subjected to Western blot analysis by indicated antibodies. (B) CFBE-CFTR cells and CFBE-ΔF508CFTR cells grown on permeable supports were transfected with 20 nM STX6 siRNA or 20 nM control siRNA as indicated. Seventy-two hours after transfection, cells were mounted on the Ussing chamber set-up and the short-circuit current was measured as described in Materials and Methods. In the graph, 50 μM genistein was added at the first arrow (black), and 1 μM CFTRinh-172 was added at the second arrow (red). Brown line, CFBE-ΔF508CFTR grown at 37°C; yellow line, CFBE-ΔF508CFTR grown at 27°C; light blue line, CFBE-ΔF508CFTR grown at 27°C and transfected with STX6 siRNA; dark blue line, CFBE-CFTR grown at 37°C; and pink line, CFBE-CFTR grown at 37°C and transfected with STX6 siRNA.