Figure 8.

TAMPs have unique dynamic behaviors at bicellular and tricellular tight junction regions. (A) Caco-2 cells were transfected with EGFP-TAMP fusion proteins, and protein dynamics at bicellular (bTJ) and tricellular (tTJ) tight junction regions were assessed by FRAP. TAMPs exhibit distinct and unique dynamic behaviors at tricellular (B–D) and bicellular (E–G) areas of the tight junction. Occludin and tricellulin are highly mobile at both regions. In contrast, mobility of both marvelD3 splice variants varied between bicellular and tricellular tight junction regions (see Table 1). Data are representative of ≥3 experiments, each in triplicate, all with similar results.