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. 2010 Apr 1;21(7):1237–1252. doi: 10.1091/mbc.E09-05-0412

Figure 1.

Figure 1.

The bni1-116 bnr1Δ mutant shows growth arrest with a small bud. (A) Temperature-sensitive growth in the bni1-116 bnr1Δ mutant. Strains were streaked onto YPDA plates, followed by incubation at 25°C for 3 d or at 35°C for 2 d. Strains were wild type (WT, YKT38), bni1Δ (YKT382), bnr1Δ (YKT390), and bni1-116 bnr1Δ (YKT503). (B) Filamentous actin structures in the bni1-116 bnr1Δ mutant. Strains were grown in YPDA medium at 18°C and then shifted to 35°C for 5 min. Cells were fixed and stained for filamentous actin with TRITC-phalloidin. Strains were wild type (YKT7) and bni1-116/bni1-116 bnr1Δ/bnr1Δ (YKT458). (C) Growth arrest with a small bud in bni1-116 bnr1Δ cells. Exponentially growing wild-type (YKT38) and bni1-116 bnr1Δ (YKT503) cells were shifted to 37°C and fixed with 3.7% formaldehyde at the indicated time point. The graph shows the percentage of cells with the bud size that was categorized as described in Materials and Methods. The right panel displays images of cells after a 160-min incubation. (D) Time course of small-bud formation after release from G1 arrest. Wild-type (YKT38) and bni1-116 bnr1Δ (YKT503) cells were arrested with α-factor as described in Materials and Methods and released into fresh medium at 37°C, followed by fixation with 3.7% formaldehyde at the indicated time point. The graph shows the percentage of cells with the bud size as in C. Right, images of cells after incubation for the indicated time periods. Bars, 5 μm.