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. 2010 Apr 1;21(7):1253–1262. doi: 10.1091/mbc.E09-10-0861

Figure 1.

Figure 1.

Bnr1p localization domains lie in the N-terminal 1-733 region. (A) Domain structures of Bnr1p and diagrams of Bnr1p constructs used in this study. G, GTPase binding domain; CC, coiled-coil domain; DAD, diaphanous autoregulatory domain; DD, dimerization domain; DID, diaphanous inhibitory domain; FH1 and FH2, formin homology domains 1 and 2; L1, Bnr1p-L1, localization region 1; Bnr1p-L2, Bnr1p localization region 2. Constructs that are localized at the bud neck are in green, constructs that are not localized at the bud neck are in red, and constructs that are not detected by immunoblotting are in black. (B) The N-terminal domain localizes Bnr1p to the bud neck. Localization of 3HA-Bnr1p-1-491 and 381-733 (under GAL1 promoter and induced with galactose for 2–3 h), as visualized by immunofluorescence with antibody to the HA-tag. Bar, 2 μm.