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Bnr1p has two distinct localization domains: Bnr1p-L1 and Bnr1p-L2. Localization of HA-Bnr1p-381-491, 1-461, 1-466 (Bnr1p-L1), 466-733 (Bnr1p-L2), 471-733 and 381-606 (under GAL1 promoter and induced with galactose for 2–3 h), as visualized by immunofluorescence with antibody to the HA-tag. Bar, 2 μm.
