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. 2010 Apr 1;21(7):1253–1262. doi: 10.1091/mbc.E09-10-0861

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Figure 3. — The localization domains are not dependent on endogenous Bnr1p, but do depend on septins. (A) Localization of 3HA-Bnr1p-L1 and 3HA-Bnr1p-L2 (under GAL1 promoter and induced with galactose for 2–3 h) in wild-type and bnr1Δ cells, as visualized by immunofluorescence with antibody to the HA-tag. Bar, 2 μm. (B) Localization of 3HA-Bnr1p-L1 and 3HA-Bnr1p-L2 (under GAL1 promoter and induced with galactose for 2–3 h) in wild type and cdc10–1 cells (1 h at 35°C), as visualized by immunofluorescence with antibody to the HA-tag. Bar, 2 μm.