Figure 5.

Bnr1p domains expressed from the endogenous promoter exhibit similar localization patterns. (A) Western blot with antibodies to GFP to show relative levels of expression of the indicated Bnr1p constructs. (B–F) Localization of Bnr1p-GFP (B), 3GFP-Bnr1p-L2 (C), 3GFP-Bnr1p-L1-L2 (D), 3GFP-Bnr1pΔL1 (E), and 3GFP-Bnr1pΔL2 (F) during the cell cycle. All constructs were expressed from the BNR1 promoter in cells also expressing Cdc3-mCherry. In each panel, the top row shows Bnr1p or Bnr1p constructs, the middle row shows Cdc3p-mCherry and the bottom row shows the merged images. The numbers indicate time points in minutes.